4 6 diamidino 2 phenylindol  (Vector Laboratories)

Journal: Atherosclerosis Plus

Article Title: Heterozygous transferrin receptor 1 deletion reduces atherosclerotic lesion formation in apolipoprotein E-deficient mice

doi: 10.1016/j.athplu.2025.12.003

Figure Lengend Snippet: Effects of heterozygous transferrin receptor 1 deletion on atherosclerotic lesion formation in apolipoprotein E-deficient mice . (A) Experimental protocol; 6 weeks-old ApoE −/− or ApoE −/− /TfR1 +/− mice were fed for 16 weeks with a high-fat diet (HFD), and representative photographs of Oil Red O-stained sections (scale bar: 500 μm) from the aortic sinus and en face whole aorta, and quantification of Oil Red O-stained area in the aortic sinus sections and en face whole aortas of ApoE −/− and ApoE −/− /TfR1 +/− mice at 16 weeks after a high-fat diet (HFD) ( n = 6–10/group). (B) Representative images of TfR1, 4-hydroxynonenal (4HNE), and CD68 staining in the aortic sinus sections (scale bar: 500 μm) in ApoE −/− and ApoE −/− /TfR1 +/− mice at 16 weeks after a high-fat diet (HFD). (C) Quantification of TfR1, 4HNE, and CD68-positive area in the aortic sinus sections of ApoE −/− and ApoE −/− /TfR1 +/− mice at 16 weeks after a high-fat diet (HFD) ( n = 6/group). (D) Representative merged image of immunofluorescence staining of CD68, TfR1, and DAPI in the aortic sinus sections (scale bar: upper-left 500, upper-right and lower-left 200, lower-right 100 μm) of ApoE −/− mice at 16 weeks after a high-fat diet. DAPI, 4′,6-diamidino-2-phenylindol. (E) Western blot analysis for aortic TfR1, transferrin, and ferritin in ApoE −/− and ApoE −/− /TfR1 +/− mice at 16 weeks after a high-fat diet (HFD) ( n = 4/group). (F) Representative images of Oil Red O, TfR1, 4HNE, and CD68 staining in the aorta sections (scale bar: 100 μm) in ApoE −/− and ApoE −/− /TfR1 +/− mice at 16 weeks after a high-fat diet (HFD). (G) Quantification of Oil Red O, TfR1, 4HNE, and CD68-positive area in the aorta sections of ApoE −/− and ApoE −/− /TfR1 +/− mice at 16 weeks after a high-fat diet (HFD) ( n = 6/group). (H) Representative images of immunofluorescence staining of CD68 and TfR1, and merged image of CD68, TfR1, and DAPI in the aortic sections (scale bar: upper-left, upper-right, and lower-left 100, lower-right 50 μm) of ApoE −/− mice at 16 weeks after a high-fat diet. (I) Body weight, serum total cholesterol (TC), low-density lipoprotein cholesterol (LDL), high-density lipoprotein cholesterol (HDL), triglycerides (TG), iron, hemoglobin, and mean corpuscular volume (MCV) in ApoE −/− and ApoE −/− /TfR1 +/− mice at 16 weeks after a high-fat diet (HFD). N = 5–6/group. Results are shown as means ± SEM. Student's t-test was used and a probability value < 0.05 was described in each Figure. ns, not significant.

Article Snippet: Nuclei were counterstained with 4’,6-diamidino-2-phenylindol (catalog # H-1800 , Vector Laboratories).

Techniques: Staining, Immunofluorescence, Western Blot

Journal: bioRxiv

Article Title: MNRR1 is required for Triple Negative Breast Cancer growth and metastasis and can be targeted by repurposed drugs

doi: 10.64898/2026.01.31.703055

Figure Lengend Snippet: S1A. Protein levels of MNRR1 are higher in HTB126 (breast cancer line) vs HTB125 (isogenic control) cells detected via immunofluorescent staining. DAPI was used to indicate cellular nuclei. Cells were imaged at 60x, and scale bar represents 50μm. S1B. Protein levels of MNRR1 are higher in 231 and 468 (breast cancer lines) vs 10A (normal breast cell line) cells, detected via immunofluorescent staining. DAPI was used to indicate cellular nuclei. Cells were imaged at 60x, and scale bar represents 50μm. S1C. Overview of the high-throughput screening process to identify inhibitors of MNRR1 using the inverted pyramid approach. S1D. Protein levels of MNRR1 are reduced in 468 cells treated with increasing concentrations of MNRR1 inhibitor as analyzed via western blot. GAPDH was probed as loading control. S1E. Level of EMT marker vimentin is reduced in 468 cells overexpressing transcriptionally defective MNRR1 (TD-R1) compared to empty vector (EV). MNRR1 was probed to confirm overexpression and GAPDH was probed as loading control. S1F. Mesenchymal marker N-cadherin is reduced in MNRR1-knockdown (R1-KD) 468 cells as analyzed via western blot. MNRR1 was probed to confirm knockdown, and tubulin was probed as loading control. S1G. ZO-1 levels are increased, and Snail levels are reduced in 231 cells overexpressing transcriptionally defective MNRR1 (TD-R1) compared to empty vector (EV). MNRR1 was probed to confirm overexpression, and Actin was probed as loading control.

Article Snippet: Cells or tissue sections were washed 3 times with PBST for 5 min each and mounted with Vectashield vibrance with DAPI (Cat. # H-1800-10, Vector Labs, Newark, CA, USA).

Techniques: Control, Staining, High Throughput Screening Assay, Western Blot, Marker, Plasmid Preparation, Over Expression, Knockdown

Journal: bioRxiv

Article Title: MNRR1 is required for Triple Negative Breast Cancer growth and metastasis and can be targeted by repurposed drugs

doi: 10.64898/2026.01.31.703055

Figure Lengend Snippet: A. Left , Protein levels of MNRR1 are higher breast tissues sections from NHB compared to NHW detected via immunofluorescent staining. DAPI was used to label cellular nuclei. Tissues were imaged at 60x, and scale bar represents 20μm. Left , Quantification of protein levels from NHB and NHW tissues (n=14, *p<0.05). B. Transcript levels of MNRR1 are higher in breast tissues from non-Hispanic Black women (NHB) compared to non-Hispanic White women (NHW). 18s rRNA was used as a housekeeping gene for the RT-qPCR analysis (n=5, *p<0.05). C: Breast tumors grow more slowly in the presence of the MNRR1 inhibitor cephalexin as detailed in Materials and Methods (n=3 in each group). D: Protein levels of MNRR1 are reduced in tumors harvested from mice treated with vehicle or cephalexin. Actin was probed as loading control. E: Quantification of MNRR1 protein levels from harvested tumors in D.

Article Snippet: Cells or tissue sections were washed 3 times with PBST for 5 min each and mounted with Vectashield vibrance with DAPI (Cat. # H-1800-10, Vector Labs, Newark, CA, USA).

Techniques: Staining, Quantitative RT-PCR, Control